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Anti -alpha Actinin/ACTN1 Rabbit pAb

貨號:GB111557

品牌:Servicebio

價格:¥650

規格: 100 μl

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Product Information

 

Description ACTN1 rabbit polyclonal
Protein full name Alpha-actinin-1
Synonyms Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, actinin, Non-muscle alpha-actinin-1, ACTN1, actinin 1 smooth muscle, Actn1a, BDPLT15, I79_004057
Immunogen KLH conjugated Synthetic peptide
corresponding to Human ACTN1
Isotype IgG
Purity Affinity purification
Subcellular location Cell membrane, Cytoplasm
Predicted MW. 103 kDa
Observed MW. 103 kDa
Uniprot ID P12814, Q7TPR4, Q9Z1P2

 


 

Applications

 

Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 spleen, skeletal muscle
IHC Mouse 1: 400-1: 800 skeletal muscle, muscle, skin, heart
IF Mouse 1: 400-1: 800 muscle, uterus

 


 

Background
 

Alpha actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. ACTN1 is a F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

 


 

Images

 


Western blot analysis of ACTN1 (GB111557) 
at dilution of 1: 1000
Lane 1: NIH3T3 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse spleen tissue lysate
Lane 5: Mouse skeletal muscle tissue lysate
Lane 6: Mouse spleen tissue lysate
Lane 7: Rat skeletal muscle tissue lysate
 

Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using ACTN1 (GB111557) at dilution of 1: 750
 

Immunohistochemistry analysis of paraffin-embedded mouse muscle using ACTN1 (GB111557) at dilution of 1: 750
 

Immunohistochemistry analysis of paraffin-embedded mouse skin using ACTN1 (GB111557) at dilution of 1: 750
 

Immunohistochemistry analysis of paraffin-embedded mouse heart using ACTN1 (GB111557) at dilution of 1: 750
 

Immunofluorescent analysis of paraformaldehyde-fixed mouse muscle using ACTN1 (GB111557) at dilution of 1: 500
 

Immunofluorescent analysis of paraformaldehyde-fixed mouse uterus using ACTN1 (GB111557) at dilution of 1: 500
 

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.
 

 

NOTE: This product is intended for research only.

 

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